We have employed fluorescent in situ hybridiation (FISH) in many of our studies to measure leukemia-specific chromosomal aberrations in the lymphocytes of exposed individuals. FISH uses fluorescent probes that bind to similar sequence chromosomes that allow detection and localization the presence or absence of specific DNA sequences.
Our latest application of OctoChrome FISH will allow us to investigate chemical effects on all 24 human chromosomes simultaneously by examining any structural aberrations, loss and gain of chromosomes, and leukemia-specific translocations and rearrangements in our sample DNA.
Escobar PA, Smith MT, Vasishta A, Hubbard AE, and Zhang L. (2007) Leukaemia-specific chromosome damage detected by comet with fluorescence in situ hybridization (comet-FISH). Mutagenesis, 22(5):321-7. PMID 17575318. [Abstract] [Full Text]
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